بررسی ویروس موزاییک یونجه در مناطق شمالی و مرکزی استان خراسان

The study of dispersion of alfalfa mosaic virus (ALMV) infection based on DAS-ELISA indicated that the fields of Alfalfa, potatoes and tomatoes from Chenaran, Ghochan, Shirvan, Mashhad, Neishaboor and Torbat Heydarieh were infected with the virus. The Statistical analysis indicated that the amount of infection did not differ in the surveyed regions and total mean of infection was 53 percent. The samples collected from the Alfalfa field of Mashhad was propagated in the Nicotiana tabacum L.cv Samsun and then virus was purified. The Mechanical inoculation of this isolate of the alfalfa mosaic virus (ALMV) induced the local lesion in Chenopodium quinoa,C. amaranticolor,Vigna unguiculata, Phaseolus vulgaris cv Redkidney and the systemic vein clearing and mosaic in Nicotiana glutinosa, N. tabacum cv. Samsun, Ocimum basilicum, Cicer arietinum and Lycopersicon esculentum. In the case of the infected Cucumis sativus, no symptoms was observed. ALMV was purified by the method of the kaiser and Robertson(1976). The virus yield was 11/05 mg per 100g of infected tissue on the basis of serological properties. This isolate of ALMV is similar to the American isolation in SDS-PAGE and Western Blot analysis,the molecular weight of the virus coat proteins were estimated at about 24000 daltons.in this regard,this isolation of ALMV is similar to the other isolates of ALMV reported elswhere.